Thermo Fisher Scientific SETD8 Monoclonal Antibody (B.540.4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

B.540.4

Immunogen

Synthetic peptide corresponding to human SET8 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

25 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/mL BSA, 0.15M NaCl

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_11004382

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

SETD8 is a histone methyltransferase that specifically monomethylates Lys-20 of histone H4. H4 Lys-20 monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. The protein mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. It is also involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.