Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Violet 480, eBioscience
마우스 및 돼지 CD117(c-Kit) 인식 단클론 항체로 Brilliant Violet 480 형광 표지. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저 사용 시 479 nm에서 방출. 조혈전구세포 및 비만세포 연구용으로 적합.
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), Brilliant Violet 480, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Species Reactivity
Mouse, Pig
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | Brilliant Violet™ 480 |
| Excitation / Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929093 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 605
- Brilliant Violet 650
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed on hematopoietic progenitor cells, mast cells, and plays a role in their proliferation and differentiation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test (100 µL final volume). Optimize antibody titration per assay.
Brilliant Violet™ 480 (BV480):
- Emits at 479 nm, excitable with 405 nm violet laser
- Not compatible with methanol-based fixation
- Recommended buffers: Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize polymer dye interactions
Laser Compatibility: Excitation 440 nm; Emission 479 nm; Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.
Target Information
KIT (c-KIT) is a proto-oncogene and type 3 transmembrane receptor for mast cell growth factor (stem cell factor). It regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations in KIT are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells, and gastrointestinal tract cajal cells. Ligand binding induces receptor dimerization and activation, triggering downstream signaling pathways such as Ras/ERK, PI3K, PLC-γ, and JAK/STAT.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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