Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 1.0 µg/test

Product Specifications

Additional Formats Available

• APC
• Biotin
• Brilliant Violet 421
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein linked chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4⁺ T cell responses to grafts, with CXCR3 knockout mice showing compromised allograft rejection responses. Expression is found on NK cells, subsets of T lymphocytes and Tregs, and preferentially on Th1-polarized cells.

The CXCR3-173 antibody affects chemotaxis in response to ligand; ligand presence eliminates staining. In vivo, antibody addition delays cardiac and pancreatic islet allograft rejection.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤ 1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.
• Fluorochrome: Brilliant Violet™ 480 (BV480), emission at 479 nm, for cytometers with 405 nm violet laser.
• Buffer Recommendation: When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75).
• Fixation Compatibility: Not compatible with methanol-based fixation.
• Excitation/Emission: 440 nm / 479 nm
• Laser: Violet Laser

Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.™

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily, mediating leukocyte trafficking, integrin activation, cytoskeletal changes, and chemotaxis. Signal transduction involves Gi-subclass G proteins. Receptor internalization and recycling regulate response duration. Expression is high in effector/memory T cells and inflamed tissues, implicating CXCR3 in inflammatory cell recruitment. It is a potential target for small-molecule antagonists for inflammatory disease treatment. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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