Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563 / 615 / 737
• Brilliant Violet 421 / 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• PE / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on intraepithelial lymphocytes, a subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC).
E-cadherin binds to CD103 and mediates lymphocyte homing to intestinal epithelium.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse lymph node cells at ≤1.0 µg/test (100 µL final volume).
  Cell number may range from 10⁵–10⁸ cells/test; titration recommended for optimal performance.

Brilliant Violet™ 480 (BV480) emits at 479 nm and is intended for use on cytometers equipped with a violet (405 nm) laser.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.

Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes and activated cells, comprising a 150 kDa alpha chain and a 120 kDa beta chain. CD103 is found on 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs, and stains few bone marrow cells.
As part of the integrin family, CD103 forms heterodimeric integral membrane proteins with beta chains. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage into disulfide-linked heavy and light chains.
Together with integrin beta7, CD103 forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. It is preferentially expressed in intestinal intraepithelial lymphocytes (IEL) and may function in adhesion and activation.
Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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