Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 625
Qdot 625 형광체가 결합된 Donkey anti-Rabbit IgG (H+L) 2차 항체로, 높은 밝기와 내광표백성을 제공. Western blot, IHC, ICC/IF, Flow cytometry에 적합. 4°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 625
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Qdot™ 625 |
| Excitation/Emission Max | 300 / 621 nm |
| Form | Liquid |
| Concentration | 1 µM |
| Purification | Purified |
| Storage Buffer | 0.05M borate, pH 8.3, with 1M betaine |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2556493 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Qdot™ nanocrystals are composed of semiconductor materials that generate exceptionally bright fluorescent particles resistant to photobleaching. When paired with appropriate optical filters, Qdot nanocrystals can be up to 50 times brighter than traditional organic dyes.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of target proteins. Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 594
- AP
- Biotin
- FITC
- HRP
- Qdot 525
- Qdot 565
- Qdot 655
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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