Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) Secondary Antibody, Qdot 605
Thermo Fisher의 Donkey anti-Mouse IgG (H+L) Qdot 605 항체는 마우스 IgG 검출용 형광 보조항체로, 높은 광도와 내광표백성을 제공합니다. Western blot, IHC, ICC/IF, Flow cytometry에 적합하며, 300/603 nm의 발광 특성을 가집니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Qdot™ 605 |
| Excitation / Emission Max | 300 / 603 nm |
| Form | Liquid |
| Concentration | 1 µM |
| Purification | Purified |
| Storage Buffer | 0.05 M borate, pH 8.3, with 1 M betaine |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2556489 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 594
- AP (Alkaline Phosphatase)
- Biotin
- FITC
- HRP
- Qdot 525
- Qdot 625
- Texas Red
- TRITC
- Custom conjugation available on request
Product Specific Information
Qdot™ nanocrystals are composed of semiconductor materials that generate exceptionally bright fluorescent particles resistant to photobleaching. When paired with the correct optical filters, Qdot nanocrystals can be up to 50 times brighter than traditional organic dyes.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of mouse antibodies. Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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