Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), APC, eBioscience

Thermo Fisher Scientific CD158a/h/g Monoclonal Antibody (HP-MA4), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.125 µg)/test
  View publication

Product Specifications

Additional Formats

• eFluor 450
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-MA4 recognizes several CD158 proteins, also known as KIR2D, specifically KIR2DL1 (CD158a), KIR2DS1 (CD158h), KIR2DS3, and KIR2DS5 (CD158g).
It does not recognize KIR2DL2 (CD158b1), KIR2DS2 (CD158j), KIR2DL4 (CD158d) or KIR2DS4 (CD158i). Crossreactivity to KIR2DL3 (CD158b2) and KIR2DL5 (CD158f) is unknown.

CD158 proteins are members of the killer cell immunoglobulin-like receptor (KIR) family of transmembrane glycoproteins expressed on NK cells and subsets of T cells.
Each KIR has an isoform with either a short (S) or long (L) cytoplasmic domain that transduces activating or inhibitory signals, respectively.
The crossreactivity of HP-MA4 antibody to KIR2DS3 and KIR2DS5 was published in 2013.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells. Recommended use at 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells.
The KIR genes are polymorphic and homologous, located on chromosome 19q13.4 within the leukocyte receptor complex (LRC).
KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by their cytoplasmic domain length (L or S).
Proteins with long cytoplasmic domains transmit inhibitory signals via ITIM motifs, while short-domain KIRs associate with TYRO protein tyrosine kinase binding protein to transmit activating signals.
Ligands for KIR proteins are subsets of HLA class I molecules, suggesting an important role in immune regulation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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