Thermo Fisher Scientific HSF1 Polyclonal Antibody
Thermo Fisher Scientific의 HSF1 Polyclonal Antibody는 인간 HSF1 단백질(425–475 aa 영역)을 인식하는 토끼 폴리클로날 항체로, WB, IHC, IP 등에 사용됩니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 1 mg/mL 농도입니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific HSF1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:5,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 425 and 475 of human Heat Shock Transcription Factor 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
All organisms respond to elevated temperatures and various environmental stresses by rapid synthesis of heat shock RNAs and proteins. Regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs).
These HSEs consist of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation upstream of heat shock genes. Although HSEs are conserved among species, HSF proteins from yeast, Drosophila, and human differ in molecular weight and show minimal immunological cross-reactivity.
In human cells, two HSFs have been identified: HSF1 and HSF2, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA binding activity. Upon stress, HSF1 is activated to a nuclear-localized trimeric form that binds DNA. Phosphorylation of HSF1 is essential for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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