Thermo Fisher Scientific HSF1 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Bovine

Target Information

All organisms respond to elevated temperatures and various environmental stresses by synthesizing heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator Heat Shock Factor (HSF), which binds to Heat Shock Response Elements (HSEs).
HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are conserved among species.
In humans, two HSFs (HSF1 and HSF2) have been identified, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists in unstressed cells as a cytoplasmic and nuclear monomer lacking DNA binding activity. Upon stress, it becomes a nuclear-localized trimer that binds DNA, and phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.