Thermo Fisher Scientific HSF1 Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 적합한 HSF1 폴리클로날 항체. 인간 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. TBS 버퍼(0.1% BSA) 내 액상 형태로 제공되며, 4°C에서 보관. 연구용으로만 사용 가능.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific HSF1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 479 and 529 of human Heat Shock Transcription Factor 1 (HSF1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Bovine
Target Information
All organisms respond to elevated temperatures and various environmental stresses by synthesizing heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator Heat Shock Factor (HSF), which binds to Heat Shock Response Elements (HSEs).
HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are conserved among species.
In humans, two HSFs (HSF1 and HSF2) have been identified, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.
HSF1 exists in unstressed cells as a cytoplasmic and nuclear monomer lacking DNA binding activity. Upon stress, it becomes a nuclear-localized trimer that binds DNA, and phosphorylation of HSF1 is required for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.