Thermo Fisher Scientific HMGB1 Recombinant Rabbit Monoclonal Antibody (1A1)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:5,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

This antibody has been tested in direct-ELISA.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein. Damaged or necrotic cells release HMGB1 into the extracellular space, where it acts as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:

• Two positively charged DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
• A negatively charged C-terminal acidic tail (30 aa)
• Nuclear localization signals at residues 28–44 and 180–185

The B box confers cytokine activity, while the A box is associated with transcription factor binding. HMGB1 mediates inflammation, monocyte migration, cytokine secretion, and T cell–dendritic cell interactions. It signals through receptors such as RAGE, TLR2, and TLR4, and is highly conserved across species.

HMGB1 levels are elevated in conditions such as:

• Sterile tissue injury
• Infection
• Endotoxemia or sepsis
• Collagen-induced arthritis
• Ischemia-reperfusion injury

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.