Thermo Fisher Scientific Phospho-p130 (Ser639) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human RBL2(Accession Q08999), corresponding to amino acid residues around phosphorylated Ser639. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:p130,uniProtId:Q08999-1,ncbiNodeId:9606,antigenRange:639,antigenLength:1139,antigenImageFileName:PA5-118748_p130_Q08999-1_Rabbit.svg,antigenImageFileNamePDP:PA5-118748_p130_Q08999-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2903248

Product Specific Information

Antibody detects endogenous levels of RBL2 only when phosphorylated at Ser639.

Target Information

RBL2 (also known as p130) or Retinoblastoma-like protein 2 is a key regulator of entry into cell division. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and (in particular) that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C leading to epigenetic transcriptional repression. It controls histone H4 Lys20 trimethylation. It probably also acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 serves as a potent inhibitor of E2F-mediated trans-activation and associates preferentially with E2F5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.