Thermo Fisher Scientific Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), PE, eBioscience

Thermo Fisher Scientific Phospho-SRC (Tyr418) Monoclonal Antibody (SC1T2M3), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 1 publication

Product Specifications

Product Specific Information

This SC1T2M3 monoclonal antibody recognizes human and mouse Src tyrosine kinase phosphorylated on tyrosine 418 (Y418). Autophosphorylation at Y418 is essential for full catalytic activity. Src is a non-receptor tyrosine kinase involved in signal transduction across diverse receptor classes including immune receptors, integrins, receptor tyrosine kinases, GPCRs, and cytokine receptors. Aberrant Src activity is implicated in various cancers.

Due to sequence homology around Src Y418, this clone is predicted to cross-react with other Src family kinases (Src, Lck, Fyn, Lyn). Specificity was confirmed by ELISA, flow cytometry, and western blotting.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-titrated for intracellular staining of normal human peripheral blood cells at 5 µL (0.25 µg)/test
• Cell Range: 10⁵–10⁸ cells/test

Staining Protocols

• Protocol A: Two-step (cytoplasmic proteins) – flexible detection of surface and intracellular proteins
• Protocol B: One-step (nuclear proteins) – ideal for transcription factors and phosphorylated proteins
• Protocol C: Two-step (Fixation/Methanol) – optimal for phospho-specific signaling discrimination

Detailed protocols available in BestProtocols®: Staining Intracellular Antigens for Flow Cytometry.

• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

SRC is a cytoplasmic tyrosine kinase and proto-oncogene regulating embryonic development, cell growth, differentiation, and apoptosis. It associates with cytoplasmic regions of various receptors for signal transduction and participates in cytoskeletal organization and cell cycle control. In humans, SRC is located on chromosome 20.

Phosphorylation of Tyr416 activates SRC, while Tyr527 phosphorylation inhibits it. Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encode the same SRC protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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