Thermo Fisher Scientific IkB alpha Monoclonal Antibody (MFRDTRK), PE, eBioscience
Human 및 Mouse IkB alpha 단백질 검출용 PE 결합 단클론 항체. Flow Cytometry에 최적화되어 있으며, 5 µL/test 사용. NF-kappaB 신호 억제 관련 연구에 적합. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific IkB alpha Monoclonal Antibody (MFRDTRK), PE, eBioscience™
Applications
- Flow Cytometry (Intracellular Staining)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MFRDTRK |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2572682 |
Product Specific Information
This MFRDTRK monoclonal antibody recognizes human and mouse IkB alpha, a member of the inhibitor family of NF-kappaB signaling. IkB alpha prevents NF-kappaB nuclear translocation by masking its nuclear localization signals, keeping it inactive in the cytoplasm. Upon stimulation (e.g., TNF alpha, IL-1, ROS), IkB kinase (IKK) phosphorylates IkB alpha, leading to ubiquitination and degradation, allowing NF-kappaB activation.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining of normal human peripheral blood cells at 5 µL (0.125 µg) per test.
Recommended Cell Number: 10⁵–10⁸ cells/test.
Staining Protocols:
- Protocol A: Two-step protocol for surface and intracellular protein detection.
- Protocol C: Two-step fixation/methanol protocol for phospho-specific signaling detection.
Refer to BestProtocols® – Staining Intracellular Antigens for Flow Cytometry for detailed procedures.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
IkB-alpha (≈40 kDa) inhibits NF-kappaB activity by binding NF-kappaB dimers in the cytosol and masking their nuclear localization sequence. Degradation of IkB-alpha, triggered by stimuli such as TNF-alpha, IL-1 beta, LPS, UV, and viral infection, releases NF-kappaB for nuclear translocation and gene activation.
The human IkB-alpha gene is located on chromosome 14q. Phosphorylation on serine residues 32 and 36 by IKK alpha (CHUK) induces degradation, enabling NF-kappaB activation. The IKK complex (IKK alpha, IKK beta, IKK gamma) is essential for this process.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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