Thermo Fisher Scientific IkB alpha Monoclonal Antibody (MFRDTRK), PE, eBioscience

Thermo Fisher Scientific IkB alpha Monoclonal Antibody (MFRDTRK), PE, eBioscience™

Applications

• Flow Cytometry (Intracellular Staining)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication

Product Specifications

Product Specific Information

This MFRDTRK monoclonal antibody recognizes human and mouse IkB alpha, a member of the inhibitor family of NF-kappaB signaling. IkB alpha prevents NF-kappaB nuclear translocation by masking its nuclear localization signals, keeping it inactive in the cytoplasm. Upon stimulation (e.g., TNF alpha, IL-1, ROS), IkB kinase (IKK) phosphorylates IkB alpha, leading to ubiquitination and degradation, allowing NF-kappaB activation.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-titrated for intracellular staining of normal human peripheral blood cells at 5 µL (0.125 µg) per test.
Recommended Cell Number: 10⁵–10⁸ cells/test.

Staining Protocols:

• Protocol A: Two-step protocol for surface and intracellular protein detection.
• Protocol C: Two-step fixation/methanol protocol for phospho-specific signaling detection.
  Refer to BestProtocols® – Staining Intracellular Antigens for Flow Cytometry for detailed procedures.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

IkB-alpha (≈40 kDa) inhibits NF-kappaB activity by binding NF-kappaB dimers in the cytosol and masking their nuclear localization sequence. Degradation of IkB-alpha, triggered by stimuli such as TNF-alpha, IL-1 beta, LPS, UV, and viral infection, releases NF-kappaB for nuclear translocation and gene activation.
The human IkB-alpha gene is located on chromosome 14q. Phosphorylation on serine residues 32 and 36 by IKK alpha (CHUK) induces degradation, enabling NF-kappaB activation. The IKK complex (IKK alpha, IKK beta, IKK gamma) is essential for this process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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