Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), Super Bright 600, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 30 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer. A small subset of lymphocytes in the thymus, spleen, and lymph nodes express CD69, and activation of T and B cells rapidly upregulates its surface expression, indicating a role in lymphocyte development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on stimulated mouse splenocytes at ≤0.25 µg/test
  • A test: 100 µL staining volume
  • Cell number: 10⁵–10⁸ cells/test
  • Optimize titration for best performance

Fluorochrome characteristics:
Super Bright 600 is a tandem dye excited by the 405 nm violet laser and emits at 600 nm. Use a 610/20 bandpass filter. Ensure instrument compatibility.

Staining recommendation:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Handling and Fixation:

• Light sensitive: Protect from light
• Fixation: Samples can be stored in
  • IC Fixation Buffer (Product # 00-8222)
  • 1-step Fix/Lyse Solution (Product # 00-5333)
    for up to 3 days at 4°C in the dark with minimal brightness loss.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, a variable percentage of cells express CD69, which is involved in lymphocyte signal transduction and activation. Expression is induced upon T lymphocyte activation, potentially contributing to proliferation and signal transduction in NK cells and platelets.
Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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