Thermo Fisher Scientific Cytokeratin 8 Monoclonal Antibody (8A5D12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8A5D12

Immunogen

Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 8,uniProtId:P05787-1,ncbiNodeId:9606,antigenRange:391-483,antigenLength:483,antigenImageFileName:MA5-15460_Cytokeratin_8_P05787-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15460_Cytokeratin_8_P05787-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10979539

Product Specific Information

MA5-15460 targets Cytokeratin 8 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15460 immunogen is purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.

MA5-15460 detects Cytokeratin 8 which has a predicted molecular weight of approximately 54kDa.

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.