Thermo Fisher Scientific Factor VIII Monoclonal Antibody (5E9B2)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

ELISA

• Tested Dilution: 1:10,000

Product Specifications

Product Specific Information

MA5-15459 targets F8 in indirect ELISA and WB applications, showing reactivity with human samples.
The immunogen used is a purified recombinant fragment of F8 expressed in E. coli.

Target Information

The F8 gene encodes coagulation factor VIII, a key component in the intrinsic pathway of blood coagulation. Factor VIII acts as a cofactor for factor IXa, which, in the presence of Ca²⁺ and phospholipids, converts factor X to its activated form Xa.

This gene produces two alternatively spliced transcripts:

1. Transcript variant 1 encodes a large glycoprotein (isoform a) that circulates in plasma and associates with von Willebrand factor in a noncovalent complex.
2. Transcript variant 2 encodes a smaller protein (isoform b) mainly consisting of the phospholipid-binding domain of factor VIIIc, essential for coagulant activity.

Defects in this gene cause hemophilia A, a common recessive X-linked coagulation disorder.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.