Thermo Fisher Scientific Human GAPD (GAPDH) Endogenous Control (VIC/MGB probe, primer limited)

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB Probe, Primer Limited)

The Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB Probe, Primer Limited) is designed as an endogenous control for relative gene expression quantification in cDNA samples when used with other gene expression assays. The probe is labeled with VIC™ dye and MGB, and primers are limited. Suitable for multiplex or singleplex PCR reactions. Compatible with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

TaqMan™ Endogenous Controls eliminate the need for extensive assay design and optimization by providing pre-designed probe and primer sets for normalization of RNA or DNA amounts in gene expression studies.

Complete Solution for Quantitative Gene Expression

These controls simplify selection of appropriate reference genes for human, mouse, rat, and eukaryotic systems, reducing trial-and-error in experimental setup.

Simple to Use

All components are QC tested and provided as a single 20X mix, compatible with universal two-step RT-PCR conditions. Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and cDNA samples to obtain sensitive and reproducible quantitative gene expression data on Applied Biosystems instruments such as 7900HT, 7300, 7500 Real-Time PCR Systems, and 7000/7700 Sequence Detection Systems.

Flexible Offering

Each endogenous control is based on 5′ nuclease chemistry. Available configurations:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM) with two unlabeled primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM) with two unlabeled primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for cDNA input differences. Ensure consistent expression across all samples. For multiplexing, the endogenous control gene should be more abundantly expressed than the target.

Multiplex vs. Singleplex PCR

TaqMan™ Endogenous Controls with VIC™-labeled probes are primer limited, allowing multiplexing with target assays when the control gene is more abundant. FAM™-labeled probes are not primer limited and are intended for singleplex use.

For Research Use Only. Not for use in diagnostic procedures.

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