Thermo Fisher Scientific CD45RO Monoclonal Antibody (UCHL1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

-

Immunohistochemistry (IHC)

-

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5-1 µg/test

View 4 publications 4 publications

Miscellaneous PubMed (Misc)

-

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Immunogen

Interleukin-2-dependent human T lymphocytes if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD45RO,uniProtId:P08575-3,ncbiNodeId:9606,antigenRange:1-1306,antigenLength:1306,antigenImageFileName:MA5-11532_CD45RO_P08575-3_House_mouse.svg,antigenImageFileNamePDP:MA5-11532_CD45RO_P08575-3_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.02 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979361

Product Specific Information

MA5-11532 targets CD45RO in WB, FACS and IHC (P) applications and shows reactivity with Human, Mouse, Rat and Non-human primate samples.

The MA5-11532 immunogen is interleukin-2-dependent human T lymphocytes.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.