Thermo Fisher Scientific Topoisomerase I Mitochondrial (TOP1MT) Recombinant Mouse Monoclonal Antibody (rTOP1MT/488)
TOP1MT 미토콘드리아 DNA 토포이소머레이스 I를 인식하는 마우스 리콤비넌트 단일클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. HEK293 발현, Protein A/G 정제, 4°C 보관.
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- 116447-MSM5-P1
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10⁶ cells |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rTOP1MT/488 |
| Immunogen | Recombinant full-length human TOP1MT protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is stable for 24 months.
- Positive Control: MCF-7 or A431 cells, Jurkat cell lysate, human heart, skeletal muscle, brain, or fetal liver tissue.
- Cellular Localization: Cytoplasm (Mitochondria)
Specificity Comments:
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. They play essential roles in DNA replication, recombination, and transcription, and are targets of many anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that relieves DNA strain during mitochondrial DNA replication. As a type IB topoisomerase, it requires divalent metals (Ca²⁺ or Mg²⁺) and an alkaline pH for optimal activity.
Target Information
This gene encodes a mitochondrial DNA topoisomerase involved in modifying DNA topology. The encoded protein, a type IB topoisomerase, catalyzes transient DNA strand breaks and rejoining to relieve supercoiling during mitochondrial DNA replication and transcription. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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