Thermo Fisher Scientific Topoisomerase I, Mitochondrial (TOP1MT) Recombinant Rabbit Monoclonal Antibody (TOP1MT, 2883R)
Recombinant rabbit monoclonal antibody against mitochondrial Topoisomerase I (TOP1MT). Validated for IHC (Paraffin). High purity Protein A/G purified, unconjugated form. Ideal for studying mitochondrial DNA topology and replication mechanisms.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested at 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | TOP1MT, 2883R |
| Immunogen | Recombinant full-length human TOP1MT protein (UniProt ID: Q969P6-1; Range: 1–601) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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