Thermo Fisher Scientific Topoisomerase (DNA) I, Mitochondrial (TOP1MT) Monoclonal Antibody (TOP1MT, 613)
인간 TOP1MT 단백질을 인식하는 마우스 단일클론 항체로, Western blot 및 IHC(파라핀 절편)에 적합합니다. 단백질 A/G로 정제된 액상 형태이며, PBS(pH 7.4)에 보존제 없이 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TOP1MT, 613 |
| Immunogen | Recombinant full-length human TOP1MT protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage conditions | -20°C or -80°C if preferred |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature (RT).
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at RT for 20 min.
Target Information
This gene encodes a mitochondrial DNA topoisomerase that modifies DNA topology. The encoded protein is a type IB topoisomerase that catalyzes transient breaking and rejoining of DNA to relieve tension and supercoiling in the mitochondrial genome during replication and transcription.
Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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