Thermo Fisher Scientific Phospho-c-Met (Tyr1234, Tyr1235) Recombinant Rabbit Monoclonal Antibody (MetY12341235-6F11), APC
Phospho-c-Met (Tyr1234, Tyr1235) 인산화 부위를 인식하는 재조합 토끼 단클론 항체입니다. Flow Cytometry에 적합하며, APC 형광단백질로 결합되어 있습니다. 높은 특이성과 재현성을 제공하며, 암 연구 및 신호전달 분석에 활용됩니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 5 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | MetY12341235-6F11 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human phospho Met |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896972 |
Additional Formats
- Unconjugated (MA5-37034)
- FITC (MA5-37036)
- PE (MA5-37035)
- Custom conjugation available upon request
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in HEK293 cells. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to large rabbit immune repertoire
Target Information
MET (c-Met) is a receptor-like tyrosine kinase involved in various cellular processes. Dysregulation of MET is associated with multiple human cancers. Upon ligand activation, MET interacts with PI3K (PIK3R1), PLCG1, SRC, GRB2, and STAT3, leading to activation of signaling pathways such as RAS-ERK, PI3K-AKT, and PLCγ-PKC.
MET functions in:
- Embryonic development (muscle, neuron, angiogenesis, kidney formation)
- Adult processes (wound healing, organ regeneration, tissue remodeling)
- Cancer progression (gastric, renal, breast cancers)
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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