Thermo Fisher Scientific PHLPP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1160-1428 of human PHLPP1 (NP_919431.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PHLPP1,uniProtId:O60346-1,ncbiNodeId:9606,antigenRange:1160-1428,antigenLength:1717,antigenImageFileName:PA5-121234_PHLPP1_O60346-1_Rabbit.svg,antigenImageFileNamePDP:PA5-121234_PHLPP1_O60346-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.94 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914806

Product Specific Information

Positive test controls include: HeLa, SH-SY5Y, Mouse testis, Mouse brain, Rat testis, Rat brain. The target is usually found in the following locations: Cell membrane, Cytoplasm, Membrane, Nucleus, Peripheral membrane protein.

Immunogen sequence: DQPSTGDASG APAVWSHGYT EASGVKNKLC VAALSVNNFC DNREALYGVF DGDRNVEVPY LLQCTMSDIL AEELQKTKNE EEYMVNTFIV MQRKLGTAGQ KLGGAAVLCH IKHDPVDPGG SFTLTSANVG KCQTVLCRNG KPLPLSRSYI MSCEEELKRI KQHKAIITED GKVNGVTEST RILGYTFLHP SVVPRPHVQS VLLTPQDEFF ILGSKGLWDS LSVEEAVEAV RNVPDALAAA KKLCTLAQSY GCHDSISAVV VQLSVTEDS

Target Information

PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.