Thermo Fisher Scientific Phospho-PLCG1 (Tyr977) Polyclonal Antibody
PLCγ1 Tyr977 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(Paraffin) 적용 가능. Human, Mouse, Rat 등 다양한 종에 반응. 고순도 정제 및 안정적 액상 형태로 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human PLCG1 (Accession P19174), corresponding to amino acid residues around phosphorylated Tyr977 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816950 |
Product Specific Information
This antibody detects endogenous levels of PLCG1 only when phosphorylated at Tyr977.
Target Information
The mammalian Phospholipase Cγ (PLCγ) family includes two closely related proteins, PLCγ1 and PLCγ2. These isozymes share core structural domains and possess additional unique domains: one PH domain, two SH2 domains, and one SH3 domain.
Upon extracellular stimulation (e.g., hormones or growth factors), receptor tyrosine kinases (RTKs) phosphorylate and activate PLCγ. Activated PLCγ catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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