Thermo Fisher Scientific Phospho-ATF1 (Ser198) Polyclonal Antibody
ATF1 단백질의 Ser198 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. 사람, 마우스, 랫트 반응성. 고순도 정제 및 안정한 PBS/glycerol 저장 buffer 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ATF1 (Accession P18846), corresponding to amino acid residues around phosphorylated Ser198. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816949 |
Product Specific Information
Antibody detects endogenous levels of ATF1 only when phosphorylated at Ser198.
Target Information
This gene encodes an activating transcription factor belonging to the ATF subfamily and bZIP (basic-region leucine zipper) family. It regulates expression of downstream target genes involved in growth, survival, and other cellular processes. The protein is phosphorylated at serine 63 in its kinase-inducible domain by several serine/threonine kinases, including cAMP-dependent protein kinase A, calmodulin-dependent kinase I/II, mitogen- and stress-activated kinase, and cyclin-dependent kinase 3 (cdk-3). Phosphorylation enhances its transactivation and transcriptional activities, promoting cell transformation. Gene fusions with FUS (chromosome 16) or EWSR1 (chromosome 22) generate chimeric proteins implicated in angiomatoid fibrous histiocytoma and clear cell sarcoma. A pseudogene exists on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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