Thermo Fisher Scientific CD25 Chimeric Recombinant Rabbit Monoclonal Antibody (7D4)
Thermo Fisher Scientific의 CD25 Chimeric Recombinant Rabbit Monoclonal Antibody (7D4)는 마우스 CD25를 인식하는 고특이성 항체로, WB, ICC/IF, Flow Cytometry 등 다양한 응용에 사용됩니다. HEK293 세포에서 발현된 재조합 단일클론 항체이며, Protein A로 정제된 액상 형태입니다.
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- MA551762
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
| Flow Cytometry (Flow) | Assay-dependent |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7D4 |
| Immunogen | The original rat IgM version of this antibody was raised by immunizing Lewis rats with HT2 cells in complete Freund’s adjuvant via intraperitoneal injection and subsequent generation of hybridomas. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
Product Specific Information
7D4 recognizes mouse CD25 at an epitope comprising amino acids 184–194 (REHHRFLASEE).
This epitope is distinct from the IL-2 binding site and does not block IL-2 binding to CD25.
Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2Rα), expressed by early progenitors of T and B lineage as well as activated mature T and B lymphocytes.
CD25 alone binds IL-2 with low affinity, but associates with CD122 (IL-2Rβ) and CD132 (common γ chain) to form the high-affinity IL-2 receptor.
Target Information
CD25 (IL2 receptor alpha chain / IL2RA) is a cytokine receptor involved in T and B lymphocyte proliferation.
The IL2 receptor complex is heterotrimeric, composed of IL2RA, IL2RB, and IL2RG.
- Homodimeric IL2RA → low-affinity receptor
- Homodimeric IL2RB → medium-affinity receptor
IL2 expression in mature thymocytes is monoallelic, representing a unique regulatory mechanism.
IL2 is mainly produced by mature T cells and acts as a growth, differentiation, and apoptosis regulator.
It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation.
Disruption of the IL2RA gene in mice leads to ulcerative colitis-like disease, indicating its role in immune response regulation.
Mutations in IL2RA are associated with interleukin 2 receptor alpha deficiency.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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