Thermo Fisher Scientific ICAM-1 Monoclonal Antibody (1H4), Biotin
ICAM-1 (CD54) 단백질을 인식하는 mouse monoclonal antibody로, Biotin으로 conjugation된 제품입니다. 다양한 응용 분야(WB, IHC, Flow Cytometry 등)에 사용 가능하며, 인간 ICAM-1에 반응합니다. 액상 형태로 제공되며, 고순도 size-exclusion chromatography로 정제되었습니다.
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Thermo Fisher Scientific ICAM-1 Monoclonal Antibody (1H4), Biotin
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 2–5 µg/mL | - |
| Functional Assay | - | 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H4 |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1072568 |
Product Specific Information
This antibody recognizes an extracellular epitope of CD54 (ICAM-1), an 85–110 kDa type I transmembrane glycoprotein that serves as a receptor for rhinovirus. It is expressed on activated endothelial cells, T and B lymphocytes, monocytes, macrophages, granulocytes, and dendritic cells. Expression is upregulated upon activation.
Target Information
ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. It has seven potential N-linked glycosylation sites and shares homology with ICAM-2 and ICAM-3. ICAM-1 binds to integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for rhinovirus. It is expressed by activated endothelial cells, epithelial cells, fibroblasts, chondrocytes, lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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