Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 737
• Brilliant Violet 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436~780)
• Request custom conjugation

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells, playing an essential role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck via its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.25 µg/test. Recommended titration for optimal performance.

Brilliant Violet™ 480 (BV480) emits at 479 nm and is designed for cytometers with a 405 nm violet laser. Verify instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Internal testing shows BV480 is not compatible with methanol-based fixation.

Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD4 antigen recognizes MHC class II molecules and acts as a co-receptor for HIV. Expressed mainly in T helper cells, and also in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut. CD4 aids early T-cell activation via association with TCR complex and Lck kinase, and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts exist (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures.

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