Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Brilliant Violet 480, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 737, 805
• Brilliant Violet 421, 650
• eFluor 450, 506, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600, 702, 780
• Request custom conjugation

Product Specific Information

The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits (gamma, delta, epsilon) are essential for assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling leading to activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume)
• Recommended: Titrate for optimal performance

Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for use with a 405 nm violet laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are immunoglobulin superfamily members encoded on chromosome 11. They associate with cytoplasmic signaling molecules via double tyrosine-based motifs.

• Expression: 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, Purkinje cells
• Not expressed: B or NK cells
• Clinical relevance: Decreased T lymphocytes in some autoimmune diseases
• Genetic location: Chromosome 11
• Defects: Associated with CD3 immunodeficiency

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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