Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Brilliant Violet 480, eBioscience
마우스 CD3 복합체를 인식하는 17A2 클론 단일클론 항체로, Brilliant Violet 480 형광체 결합. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 405 nm 레이저 사용 장비에 적합. 0.5 µg/test로 사용 권장, 4°C 암소 보관.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (17A2), Brilliant Violet 480, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 17A2 |
| Conjugate | Brilliant Violet™ 480 |
| Excitation / Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925603 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 737, 805
- Brilliant Violet 421, 650
- eFluor 450, 506, 660
- FITC
- Functional Grade
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600, 702, 780
- Request custom conjugation
Product Specific Information
The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits (gamma, delta, epsilon) are essential for assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 17A2 to CD3 initiates intracellular signaling leading to activation and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.5 µg/test (100 µL final volume)
- Recommended: Titrate for optimal performance
Brilliant Violet™ 480 (BV480) emits at 479 nm and is suitable for use with a 405 nm violet laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:
- Super Bright Complete Staining Buffer (Product # SB-4401-42)
- Brilliant Stain Buffer™ (Product # 00-4409-75)
Note: BV480 is not compatible with methanol-based fixation.
Excitation: 440 nm; Emission: 479 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are immunoglobulin superfamily members encoded on chromosome 11. They associate with cytoplasmic signaling molecules via double tyrosine-based motifs.
- Expression: 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, Purkinje cells
- Not expressed: B or NK cells
- Clinical relevance: Decreased T lymphocytes in some autoimmune diseases
- Genetic location: Chromosome 11
- Defects: Associated with CD3 immunodeficiency
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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