Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 480, eBioscience

Applications

• Flow Cytometry (Flow): Tested dilution ≤ 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated, Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–786)
• eFluor, FITC, Functional Grade, NovaFluor, PE, PE-Cyanine series, PerCP series, Super Bright series
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with mouse CD8a (32–34 kDa), expressed as CD8αβ heterodimer or CD8αα homodimer. CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤ 0.5 µg/test (in 100 µL final volume)
• Recommended Cell Number: 10⁵–10⁸ cells/test (empirically determined)
• Fluorochrome Information: Brilliant Violet™ 480 (BV480), excitation 440 nm / emission 479 nm, compatible with 405 nm violet laser
• Fixation Compatibility: Not compatible with methanol-based fixation

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein involved in signal transduction and T cell differentiation. It is expressed on T cells as disulfide-linked CD8αβ heterodimers or CD8αα homodimers. CD8 acts as a co-receptor with TCR for MHC class I molecules, aiding in cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs contribute to pathogen and tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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