Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Violet 480, eBioscience
마우스 CD8a 인식용 단클론 항체로, Brilliant Violet 480 형광체 결합. 유세포분석(Flow Cytometry)에 최적화되어 있으며 0.5 µg/test 권장. 4°C 암소 보관, 메탄올 고정 부적합. 다양한 형광체 결합 형식으로 제공.
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Applications
- Flow Cytometry (Flow): Tested dilution ≤ 0.5 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 480, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 53-6.7 |
| Conjugate | Brilliant Violet™ 480 |
| Excitation / Emission Max | 437 / 478 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2921033 |
Additional Formats Available
- Unconjugated, Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780, Biotin, Brilliant UV series (395–805), Brilliant Violet series (421–786)
- eFluor, FITC, Functional Grade, NovaFluor, PE, PE-Cyanine series, PerCP series, Super Bright series
- Request custom conjugation
Product Specific Information
The 53-6.7 monoclonal antibody reacts with mouse CD8a (32–34 kDa), expressed as CD8αβ heterodimer or CD8αα homodimer. CD8 binds to MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T cell development and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Flow cytometric analysis of mouse splenocytes, ≤ 0.5 µg/test (in 100 µL final volume)
- Recommended Cell Number: 10⁵–10⁸ cells/test (empirically determined)
- Fluorochrome Information: Brilliant Violet™ 480 (BV480), excitation 440 nm / emission 479 nm, compatible with 405 nm violet laser
- Fixation Compatibility: Not compatible with methanol-based fixation
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use:
- Super Bright Complete Staining Buffer (Product # SB-4401-42)
- Brilliant Stain Buffer™ (Product # 00-4409-75)
Trademark Notice: BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein involved in signal transduction and T cell differentiation. It is expressed on T cells as disulfide-linked CD8αβ heterodimers or CD8αα homodimers. CD8 acts as a co-receptor with TCR for MHC class I molecules, aiding in cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs contribute to pathogen and tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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