Thermo Fisher Scientific AP2S1 Polyclonal Antibody

Applications

• Western Blot (WB): Tested dilution 1:200–1:1,000

Product Specifications

Product Specific Information

This antibody detects endogenous levels of total AP2S1 protein.

Target Information

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via vesicles in different membrane traffic pathways. These complexes serve as vesicle coat components involved in cargo selection and vesicle formation.
AP-2 participates in clathrin-dependent endocytosis, facilitating incorporation of cargo proteins into clathrin-coated vesicles (CCVs) that fuse with early endosomes. The clathrin lattice acts as a mechanical scaffold but does not bind membranes directly; instead, AP complexes connect clathrin to membrane proteins and lipids, enabling cargo sorting.
AP-2 also functions as a cargo receptor for receptor-mediated endocytosis and plays a role in recycling synaptic vesicle membranes. It recognizes Y-X-X-[FILMV] and [ED]-X-X-X-L-[LI] signal motifs in cytosolic tails of transmembrane cargo molecules and may contribute to ARF6-regulated, non-clathrin trafficking pathways.
The AP-2 alpha and sigma subunits are involved in motif recognition and may also contribute to extracellular calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.