Thermo Fisher Scientific PTGER3 Monoclonal Antibody (5F5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Rat

Published species

Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

5F5

Immunogen

Recombinant human EP3 Receptor

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS

Contains

0.08% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1086853

Product Specific Information

A suggested positive control for this product is porcine brain lysate.

Target Information

CD172a, also known as signal-regulatory protein alpha, is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and haematopoietic stem cells. CD172a acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. Ligand binding of CD172a to integrin-associated protein CD47, results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of CD172a, mediating the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. These then act as regulators of cellular function, through dephosphorylation of specific substrates. Ligation of CD172a with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.