Thermo Fisher Scientific MEK1 Polyclonal Antibody
MEK1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC, ELISA 등에 사용 가능. Human 및 Mouse 반응성. Affinity 정제, 0.2 mg/mL 농도, PBS/glycerol buffer. 연구용으로 세포 신호전달 및 MAPK 경로 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1–100 of human MEK1 (NP_002746.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913610 |
Product Specific Information
Positive test controls include:
- Mouse lung
- Mouse stomach
- Mouse brain
Subcellular localization: Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, Centrosome, Cytoskeleton, Microtubule organizing center, Spindle pole body.
Immunogen sequence:
MPKKKPTPIQ LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation.
MAP2K1 is activated by a wide variety of growth factors and cytokines and also by membrane-dependent depolarization and calcium influx.
MEK1 is activated by phosphorylation of serine 218 and 222 residues by Raf1 and is involved in stress response, apoptosis, and proliferative induction by cytokines.
Mutations in the gene can lead to cardiofaciocutaneous syndrome.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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