Thermo Fisher Scientific CD101 Monoclonal Antibody (BB27), eBioscience
인간 CD101(V7 항원)을 인식하는 단클론 항체로, 유세포분석, 면역침강, 동결조직 면역조직화학에 적합합니다. 세포 활성화 연구 및 T세포 반응 조절 연구에 활용됩니다. 고순도(>90%) 항체로 0.5 mg/mL 농도의 액상 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC-F) | Assay-Dependent | |
| Flow Cytometry (Flow) | 0.5 µg/test | Tested on human peripheral blood cells |
| Immunoprecipitation (IP) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BB27 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2864956 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
The monoclonal antibody BB27 reacts with human CD101 (V7 antigen), a member of the Ig superfamily. CD101 is a disulfide-linked homodimer expressed on monocytes, granulocytes, and dendritic cells (Langerhans-like cells expressing HLA-DR, CD1a, CD1c). It is also expressed on T lymphocytes, where it plays a role in cell activation. CD101⁺CD28⁺ cells are highly responsive to CD28 signaling. In combination with anti-CD28 or suboptimal levels of anti-CD3, anti-CD101 can enhance proliferation, suggesting an activating role.
The BB27 antibody has been shown to inhibit T-cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. In mice, CD101 is expressed on a subpopulation of regulatory T cells (CD4⁺, CD25⁺, Foxp3⁺) with high suppressor activity. In human PBMCs, CD101 staining is observed in approximately 30% of Foxp3⁺ cells, though enhanced suppressor activity has not been confirmed in humans.
Applications Reported: Flow cytometry, immunoprecipitation, and immunohistochemistry (frozen tissue).
Applications Tested: Tested by flow cytometric analysis of human peripheral blood cells (≤0.5 µg/test).
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal assay performance.
Target Information
CD101 (V7 antigen) is a disulfide-linked homodimer of unknown function, expressed on monocytes, granulocytes, dendritic cells, and T lymphocytes. Its expression on T cells is associated with activation responses. CD101⁺CD28⁺ T cells show enhanced CD28-mediated signaling, and anti-CD101 can augment proliferation when combined with anti-CD28 or suboptimal anti-CD3. In mice, CD101 marks a subset of regulatory T cells with strong suppressor activity, while in humans, it is expressed on about 30% of Foxp3⁺ PBMCs without confirmed enhanced suppressor function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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