Thermo Fisher Scientific H2BK12ac Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1:1,000

-

ChIP assay (ChIP)

0.5-1 µg

-

ChIP-sequencing (ChIP-Seq)

Assay-dependent

-

Peptide Array (Array)

1:2,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH-conjugated synthetic peptide corresponding to human the region of histone H2B containing the acetylated lysine 12 (H2BK12ac). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H2BK12ac,uniProtId:P62807-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_P62807-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_P62807-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:H2BK12ac,uniProtId:Q93079-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q93079-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q93079-1_Rabbit_PDP.jpeg,sortOrder:10},{targetFamily:H2BK12ac,uniProtId:Q99877-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q99877-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q99877-1_Rabbit_PDP.jpeg,sortOrder:11},{targetFamily:H2BK12ac,uniProtId:Q99879-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q99879-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q99879-1_Rabbit_PDP.jpeg,sortOrder:12},{targetFamily:H2BK12ac,uniProtId:Q99880-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q99880-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q99880-1_Rabbit_PDP.jpeg,sortOrder:13},{targetFamily:H2BK12ac,uniProtId:O60814-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_O60814-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_O60814-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:H2BK12ac,uniProtId:P06899-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_P06899-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_P06899-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:H2BK12ac,uniProtId:P23527-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_P23527-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_P23527-1_Rabbit_PDP.jpeg,sortOrder:4},{targetFamily:H2BK12ac,uniProtId:P33778-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_P33778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_P33778-1_Rabbit_PDP.jpeg,sortOrder:5},{targetFamily:H2BK12ac,uniProtId:P57053-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_P57053-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_P57053-1_Rabbit_PDP.jpeg,sortOrder:6},{targetFamily:H2BK12ac,uniProtId:Q16778-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q16778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q16778-1_Rabbit_PDP.jpeg,sortOrder:7},{targetFamily:H2BK12ac,uniProtId:Q5QNW6-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q5QNW6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q5QNW6-1_Rabbit_PDP.jpeg,sortOrder:8},{targetFamily:H2BK12ac,uniProtId:Q8N257-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-40100_H2BK12ac_Q8N257-1_Rabbit.svg,antigenImageFileNamePDP:PA5-40100_H2BK12ac_Q8N257-1_Rabbit_PDP.jpeg,sortOrder:9}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.82 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.05% sodium azide, 0.05% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2608354

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.