Thermo Fisher Scientific Nestin Polyclonal Antibody
Rabbit polyclonal antibody against Nestin for WB and ICC/IF applications. Reacts with human, mouse, and rat. High specificity for stem/progenitor and neural cells. Supplied as liquid, unconjugated form, 1.78 mg/mL, stored at 4°C or -20°C.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Nestin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.78 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792894 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%).
- Positive Control: SK-N-SH, IMR32, SH-SY-5Y, mouse brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin serves as a superior angiogenic marker for evaluating neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages and in enhancing the therapeutic potential of human neural stem/progenitor cells for CNS disease or injury treatment.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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