Thermo Fisher Scientific GST Polyclonal Antibody, DyLight 549
GST 단백질을 인식하는 토끼 다클론 항체로 DyLight 549 형광 표지. Western blot, ICC/IF, ELISA에 적합. 높은 특이성과 민감도 제공. Schistosoma japonicum 유래 GST 면역원 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length GST isolated from Schistosoma japonicum |
| Conjugate | DyLight™ 549 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if solution is not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
Assay Information:
Immunoelectrophoresis showed a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase (Schistosoma japonicum). Cross-reactivity with GST from other sources may occur but has not been specifically determined.
Target Information
GST (Glutathione S-transferase) is a 26 kDa protein found in both eukaryotes and prokaryotes, catalyzing various reactions. It is commonly used in the GST gene fusion system to facilitate purification of fusion proteins via glutathione affinity. GST-fusion proteins are valuable tools for studying direct protein–protein interactions. The GST gene fusion system has been incorporated into numerous expression vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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