Thermo Fisher Scientific PRAS40 Polyclonal Antibody
PRAS40 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat 반응성. 고순도 Affinity Chromatography 정제, 액상 형태, -20°C 보관.
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Thermo Fisher Scientific PRAS40 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 150–256 of human AKT1S1/PRAS40 (NP_001092103.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.35 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914550 |
Product Specific Information
- Positive test controls: Mouse heart, Rat heart
- Localization: Cytoplasm, cytosol
- Immunogen sequence:
STDDGSLSEE TPAGPPTCSV PPASALPTQQ YAKSLPVSVP VWGFKEKRTE ARSSDEENGP PSSPDLDRIA ASMRALVLRE AEDTQVFGDL PRPRLNTSDF QKLKRKY
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals.
AKT1S1 (PRAS40) is a proline-rich substrate of AKT1 and plays a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
It is also a substrate and potential regulator of mTOR, which controls cell growth and cycle, acting as a negative regulator of autophagy.
Treatment with IGF1 can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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