Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Biotin, Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 42 publications 42 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 17 publications 17 publications

Flow Cytometry (Flow)

0.25 µg/test

View 239 publications 239 publications

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

-

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

M1/70

Conjugate

Biotin , Functional Grade Biotin , Functional Grade Biotin , Functional Grade

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469750

Product Specific Information

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.

Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. The M1/70 antibody is not recommended for immunohistochemical staining of formalin-fixed paraffin embedded tissue. M1/70 has also been reported in in vitro blocking of CD11b function.

Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.

Storage and handling: Use in a sterile environment.

Target Information

CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.