Thermo Fisher Scientific NFIB Monoclonal Antibody (1H4)
NFIB 단백질을 인식하는 Mouse monoclonal antibody(1H4)로, WB, IHC(P), Flow Cytometry에 적합. Human, Mouse, Rat 시료에 반응. Protein G 정제, 2 mg/mL 농도, PBS buffer에 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H4 |
| Immunogen | Recombinant full length protein corresponding to human NFIB/NF1B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911533 |
Product Specific Information
- Positive Control: SH-SY-5Y, rat heart tissue, human prostate tissue, human pancreas tissue, mouse liver tissue lysate
- Subcellular Location: Nucleus
Target Information
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain and activates transcription through a proline-rich, carboxy terminal transactivation domain. The NF-1 protein recognizes and binds the adenovirus type 2 promoter and activates transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element is found in the upstream promoter region of various eukaryotic genes, including Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein, and the Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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