Thermo Fisher Scientific Cytokeratin 5/6 Monoclonal Antibody (A2A12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A2A12

Immunogen

Synthetic peptide within Human Cytokeratin 5 aa 340-380 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cytokeratin 5/6,uniProtId:P02538-1,ncbiNodeId:9606,antigenRange:340-380,antigenLength:564,antigenImageFileName:MA5-42395_Cytokeratin_56_P02538-1_House_mouse.svg,antigenImageFileNamePDP:MA5-42395_Cytokeratin_56_P02538-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911536

Product Specific Information

Positive Control: HT-29 cell, human skin tissue, human tonsil tissue, human esophagus tissue, A431 cell.

Subcellular Location: Cytoskeleton.

Target Information

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.