Thermo Fisher Scientific Cytokeratin 5/6 Monoclonal Antibody (A2A12)
인체 Cytokeratin 5/6 단백질을 인식하는 Mouse monoclonal 항체. Western blot, IHC(P), Flow cytometry에 사용 가능. 단백질 A로 정제된 액상형 항체로 2 mg/mL 농도. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A2A12 |
| Immunogen | Synthetic peptide within Human Cytokeratin 5 (amino acids 340–380) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.4) with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911536 |
Product Specific Information
- Positive Control: HT-29 cell, human skin tissue, human tonsil tissue, human esophagus tissue, A431 cell
- Subcellular Location: Cytoskeleton
Target Information
The protein encoded by this gene is a member of the keratin gene family. Type II cytokeratins consist of basic or neutral proteins arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12–q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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