Thermo Fisher Scientific HIF1AN Polyclonal Antibody
HIF1AN 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF에 사용 가능. 인간에 반응하며, 예측 반응종은 Mouse, Rat, Zebrafish 등. 항원 친화 크로마토그래피로 정제되어 고순도 확보. 연구용으로만 사용.
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Thermo Fisher Scientific HIF1AN Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 78–349 of HIF1AN (Uniprot ID# Q9NWT6) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2552204 |
Product Specific Information
- Recommended positive controls: HCT116, MCF-7
- Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (86%), Xenopus laevis (90%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
HIF1AN hydroxylates HIF-1 alpha at Asn-803 in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor; under normoxic conditions, hydroxylation prevents interaction of HIF-1 with transcriptional coactivators such as Cbp/p300-interacting transactivator.
Involved in transcriptional repression through interaction with HIF1A, VHL, and histone deacetylases.
Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A, and other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively.
Negatively regulates NOTCH1 activity (accelerating myogenic differentiation) and positively regulates ASB4 activity (promoting vascular differentiation).
[UniProt]
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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