Thermo Fisher Scientific E2F1 Monoclonal Antibody (16G7)
E2F1 단백질을 인식하는 마우스 단일클론 항체로, Western blot, ICC/IF, IP에 사용 가능. 인간과 생쥐 시료에 반응하며, Protein G로 정제된 액상 형태. 세포주기 조절 및 종양 억제 단백질 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16G7 |
| Immunogen | Region encoding the DNA binding domain (amino acids 244–264) of E2F-1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.86 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787214 |
Product Specific Information
- Keep as concentrated solution.
- Positive Control: Jurkat cells.
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain embedded within the transactivation domain
E2F1, along with E2F2 and E2F3, contains an additional cyclin binding domain. It binds preferentially to retinoblastoma protein (pRB) in a cell-cycle-dependent manner and can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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