Thermo Fisher Scientific Influenza A M2 Monoclonal Antibody (14C2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 14 publications 14 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

10 µg/mL

-

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

14C2

Immunogen

M2 protein from A/WSN/33-infected CV1 cell lysate.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325043

Product Specific Information

MA1-082 detects the N-terminal of the influenza A virus M2 protein.

MA1-082 has been successfully used in Western blot, Immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~15 kDa protein representing the M2 protein. MA1-082 has demonstrated the ability to immunoprecipitate M2 from [35s] cysteine-labeled WSN-infected cells.

MA1-082 has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain.

The MA1-082 immunogen is M2 protein from A/WSN/33-infected CV1 cell lysate.

Target Information

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.