Thermo Fisher Scientific CD11b Polyclonal Antibody
Thermo Fisher Scientific의 CD11b Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 항체입니다. Western blot, IHC, Flow Cytometry에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS와 트레할로스를 포함한 동결건조 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human CD11b recombinant protein (Position: N129–E336) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746647 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3).
CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, subsets of NK cells and dendritic cells, and microglia in the brain.
It functions as a receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X, playing a critical role in cell-cell and cell-matrix interactions.
Expression levels vary by tissue and increase during monocyte maturation. It is widely used as a microglial marker in nervous tissue.
Diseases associated with CD11b dysfunction include systemic lupus erythematosus and ITGAM-related susceptibility to lupus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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