Thermo Fisher Scientific CD21 Monoclonal Antibody (CR2, 2754)
인간 CD21 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. 비결합형 액상 형태로 제공되며, Protein A/G 정제 및 PBS 버퍼에 보관. 4°C에서 안정적으로 저장 가능.
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Thermo Fisher Scientific CD21 Monoclonal Antibody (CR2, 2754)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CR2, 2754 |
| Immunogen | Recombinant fragment (around aa 142–240) of human CR2 (CD21) protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD21 (complement receptor 2, CR2, C3D receptor, EBV receptor) binds C3 complement fragments, particularly breakdown fragments covalently attached to complement-activating surfaces or antigens.
It plays a critical role in:
- Uptake and retention of immunocomplexes
- Survival of memory B cells
- Development and maintenance of humoral immune responses to T-dependent antigens
CD21 also serves as a receptor for Epstein-Barr virus (EBV) and mediates prion targeting to follicular dendritic cells, accelerating neuroinvasion after peripheral exposure.
A soluble form (sCD21) is shed from lymphocytes and retains ligand-binding ability.
CD21 interacts with CD23 and forms a signaling complex with CD19, CD81, and Leu13, contributing to B cell activation.
Genetic variations in CD21 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).
Alternative splicing generates multiple isoforms of CD21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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