Thermo Fisher Scientific H4K5ac Polyclonal Antibody
H4K5ac Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하며 Western blot, IHC, ELISA, ChIP 등 다양한 응용에 적합합니다. Rabbit IgG 폴리클로날 항체로, 액상 형태이며 고순도 친화 크로마토그래피로 정제되었습니다. -20°C에서 보관하며 연구용으로 사용됩니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of Chromatin |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human Histone H4 (NP_0010292491) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.14 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806015 |
Product Specific Information
- Positive Samples: HeLa, C6
- Cellular Location: Chromosome, Nucleus
- Immunogen sequence: MSGRGKGGKG LGK
Target Information
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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