Thermo Fisher Scientific E-cadherin Monoclonal Antibody (4A2C7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 28 publications 28 publications

Immunohistochemistry (IHC)

2-10 µg/mL

View 36 publications 36 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 52 publications 52 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 16 publications 16 publications

Immunoprecipitation (IP)

2-5 µg

-

ChIP assay (ChIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 52 publications 52 publications

Product Specifications

Species Reactivity

Human

Published species

Avian, Dog, Hamster, Human, Mouse, Non-human primate, Pig

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4A2C7

Immunogen

Recombinant MBP fusion protein corresponding to the cytoplasmic domain of human E-cadherin

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533118

Product Specific Information

This antibody is specific for E-cadherin but does show some minor cross-reactivity with P-cadherin.

Formalin-fixed, paraffin embedded tissue sections required a Heat Induced Epitope Retrieval (HIER) step prior to staining with this antibody in an IHC application.

Target Information

E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.