Thermo Fisher Scientific H3K36me1 Polyclonal Antibody
H3K36me1 단일메틸화 히스톤 H3에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, ChIP, Dot blot 등 다양한 응용에 사용 가능. 고순도 친화 크로마토그래피 정제, -20°C 보관, 인간 및 다종 교차반응 확인.
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Thermo Fisher Scientific H3K36me1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| ChIP assay (ChIP) | 2–5 µg/1x10⁶ cells |
| Dot blot (DB) | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Monomethyl Lys36]; affinity purified antibody prepared from rabbit serum immunized with synthetic monomethylated peptide surrounding Lysine 36 of human Histone H3.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for long-term storage.
- Avoid repeated freeze/thaw cycles.
- Centrifuge if not clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
- Anti-Histone H3 [Monomethyl Lys36] reacts with human Histone H3.2.
- Cross-reactivity suggested with Human, Mouse, and C. elegans; predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Target Information
Histone H3 is a DNA-binding protein found in chromatin of all eukaryotic cells. Together with other core histones, it forms nucleosomes and plays a central role in:
- Transcription regulation
- DNA repair and replication
- Chromosomal stability
The N-terminal tail of histone H3 undergoes various post-translational modifications, including methylation, acetylation, and phosphorylation, influencing chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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