Thermo Fisher Scientific H3R17me2a Polyclonal Antibody
Histone H3 비대칭 이메틸화 Arg17을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ChIP 등 다양한 응용에 적합합니다. Human, Mouse, C. elegans 등 여러 종에 반응하며, Affinity chromatography로 정제된 고순도 항체입니다.
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Thermo Fisher Scientific H3R17me2a Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ChIP assay (ChIP) | 2–5 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Chicken, Fruit fly, Human, Mouse, Plant, Rat, Xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 [Asym-dimethyl Arg17] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic asymmetric dimethylated peptide surrounding Arginine 17 of human Histone H3.2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 30% glycerol, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Anti-Histone H3 [Asym-dimethyl Arg17] reacts with human Histone H3.2.
BLAST analysis suggests cross-reactivity with Human, Mouse, and C. elegans.
Predicted to react with Rat, Chicken, Xenopus, Drosophila, and Plant based on 100% sequence homology.
Cross-reactivity with Histone H3 from other sources has not been determined.
Target Information
Histone H3 is one of the DNA-binding proteins found in chromatin of all eukaryotic cells. Together with four core histone proteins, H3 binds DNA to form the nucleosome structure.
Histones play key roles in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histones can alter chromatin structure or facilitate transcription factor binding.
The N-terminal tail of Histone H3 undergoes various modifications such as methylation, acetylation, and phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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